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Size (kb): 13.8000001907348600

Vector: pGPTV-BLEO (plasmid)

Construction: pBIN19, pBI101, pBIB-HYG

Marker(s):kanR

Construct size (kb): 13.80000019073486

Features: marker(s): kanR
replicon: RK2

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--13.5; BglII--8.2, 5.6; BamHI--13.5.

Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.6; EcoRI--11.6; SstI--11.9.

Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced.

The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - XbaI - HindIII - P35S - ble - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR.

Temperature: 37.0°C