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<i>Clostridioides difficile</i> (Prevot) Lawson et al.
<i>Clostridioides difficile</i> (Prevot) Lawson et al.
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貨期:
編號:B243240
品牌:Mingzhoubio

標準菌株
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DNA
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產品名稱 Clostridioides difficile (Prevot) Lawson et al.
商品貨號 B243240
Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations W1194
Application
Enteric Research
Isolation
Feces of patient with pseudomembranous colitis, Belgium.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties Serogroup A
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol&reg; no
Genotype Toxinotype: 0
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Toxinotype 0
Ribotype 005
Toxins
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Medium ATCC® Medium 2107: Modified Reinforced Clostridial
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor M Delmee
References

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

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