Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 4.6; EcoRI--10.2, 4.4; PstI--4.3, 4.0, 2.5 (doublet), 0.9; SalI--14.0; HindIII--7.0 (doublet). The insert contains the following restriction sites (nt position according to the published sequence): PstI--1843, 2786, 5318, 9763; HindIII--2775; BamHI--5462. This is the EcoRI fragment extending from nt 1 to 9906 of the published sequence subcloned from lambda33. Contains exons 1-7 and an Alu repeat. The polymorphic site is at nt 9494 of the published sequence. |