Gene product: interferon-induced protein 54(interferon, alpha-inducible protein (54kD, ISG-54K, IFI-54K), interferon-stimulated gene, 54KDa protein) [G10P2]
Insert Size (kb)
15.0
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Insert contains the following sites separated by (bp)(approx): EcoRI- 50- HindIII- 2970- HindIII- 3630- EcoRI- 124- EcoRI- 375- EcoRI- 120- HindIII- 440- EcoRI- 909- EcoRI- 770- HindIII- 1430- EcoRI...(not the complete insert).
Restriction digests of the clone give the following sizes (kb): XbaI--19.5, 15.0, 6.4 (doublet); HindIII--21.0, 7.4, 6.0, 5.4 (doublet), 1.0; SalI--uncut; EcoRI--19.5, 10.7, 6.7 (doublet), 3.4; BamHI--28.0, 7.2, 5.8, 4.2, 1.6, 0.45.
Region from nt -104 to -90 acts as an interferon-inducible enhancer.
Contains the RNA initiation site, exons 1 and 2 plus intervening sequence, and polyA signal of human ISG-54K.
The protein predicted from the DNA sequence has a possible limited homology at the amino acid level with murine Mx protein.
References
Levy D, et al. Interferon-stimulated transcription: isolation of an inducible gene and identification of its regulatory region. Proc. Natl. Acad. Sci. USA 83: 8929-8933, 1986. PubMed: 3466167
