產(chǎn)品名稱 |
Influenza A virus (H3N2) |
商品貨號(hào) |
B162800 |
Classification |
Orthomyxoviridae, Alphainfluenzavirus |
Agent |
Influenza A virus (H3N2) |
Strain |
A/California/2/2014 |
Applications |
Respiratory research Vaccine development |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 0.5 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
MDCK cells (ATCC® CCL-34™) |
Growth Conditions |
Temperature: 35°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 75% of the monolayer. |
Name of Depositor |
D Wadford, California Department of Public Health |
Source |
Isolated from a human in California, USA on January 16, 2014. |
Year of Origin |
2014 |