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pSH22.o
pSH22.o
規(guī)格:
貨期:
編號:B162640
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSH22.o
商品貨號 B162640
Designations pSH22.o
Species Homo sapiens, human
Depositors IE Scheffler
Vector
Construct size (kb): 5.5
Insert
DNA: genomic
Insert lengths(kb): 2.75
Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, single copy [D1S*3]
Insert Size (kb) 2.75
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
The insert contains the following sites separated by (bp)(approx): SalI- 300- SacI- 200- BamHI- 800- BamHI- 375- SphI- 675- PstI- 450- HindIII.
Restriction digests of the clone give the following sizes (kb): HindIII/SalI--2.75 doublet; PstI--3.4, 2.1; EcoRI--5.5; BamHI--4.4, 0.8, 0.4; XbaI--5.5.
This insert is closely linked to the centromere of human chromosome 1.
This probe detects a band of 7.4 kb in EcoRI-digested genomic DNA from human cell line HT1080 and in several human-hamster hybrids that contain an intact human chromosome 1.
The insert was derived from a minichromosome which includes the centromere of human chromosome 1.
References

Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415

Solus J, et al. Characterization of single-copy probe from vicinity of centromere of human chromosome 1. Somatic Cell Mol. Genet. 14: 381-391, 1988. PubMed: 2840744

Immo E Scheffler, personal communication

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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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