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Distribution host: Escherichia coli DH5alpha

Size (kb): 10.8999996185302700

DESCRIPTION OF VECTOR:
Intact vector size: 10.900
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40

Vector: pCMVlacZII (plasmid)

Promoters: Promoter CMV

Marker(s):neoR

Construct size (kb): 10.89999961853027

Features: marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40

reporter construct

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0.

Shuttle reporter plasmid permitting visual detection of activity by histochemical staining.

Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ.

The order of the major features in this plasmid is: CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori.

Temperature: 37.0°C