亚洲国产成人久久综合一区国产成人AV一区二区三区中文精品字幕网久久久久,亚洲人成网站18禁止久久影院,A真人一级无码毛片精品国产一区二区三区,91精品国产综合久久久久久一区黄网无码,91久久久无码国产精品免费不卡,国产欧美日本韩高清视频一区二区三区激情在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > EL4-Luc2
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
EL4-Luc2
EL4-Luc2
規(guī)格:
貨期:
編號:B161693
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EL4-Luc2
商品貨號 B161693
Organism Mus musculus, mouse
Tissue blood
Product Format frozen 1.0 mL
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain C57BL/6N
Applications Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drugs. It also can be used in cell-based assays for cancer research.
Comments This luciferase expressing cell line was derived from parental line (ATCC TIB-39) by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in blasticidin (4 μg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro.
Complete Growth Medium

The base medium for this cell line is ATCC-formulated Modified Eagle's Medium (DMEM, ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:

  • Horse serum (FBS; ATCC 30-2040) to a final concentration of 10%
  • Blasticidin to a final concentration of 4 μg/mL
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 to 4.0 x 10 5 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal: every 2 to 3 days (depending on cell density).
Cryopreservation Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial ≥ 1.0 x 106 cells
Volume 1.0 mL
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Functional Tests Luciferase activity: signal to noise ≥ 1,000 RLUs
In Vitro Luminesence: 20,000 photons/cell/sec, subject to imaging and culturing conditions
Population Doubling Time approximately 19 hrs
Name of Depositor ATCC
Year of Origin 2018
References

Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337

Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252

Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344

Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916

Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636

Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
确山县| 柘荣县| 华蓥市| 松滋市| 凤山县| 平谷区| 无极县| 山西省| 安乡县| 阜宁县| 仲巴县| 丹棱县| 蓝山县| 清丰县| 北川| 逊克县| 丹寨县| 安国市| 隆林| 毕节市| 大悟县| 东阿县| 漳平市| 高雄市| 辽阳县| 项城市| 余江县| 霍邱县| 诏安县| 延安市| 大新县| 英德市| 枣强县| 安岳县| 苏尼特左旗| 海原县| 思茅市| 宣汉县| 南康市| 修武县| 湖州市|