產(chǎn)品名稱(chēng) |
NCI-H1568 [H1568] |
商品貨號(hào) |
B161439 |
Organism |
Homo sapiens, human |
Tissue |
lung; derived from metastatic site: lymph node |
Product Format |
frozen |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
adenocarcinoma; non-small cell lung cancer |
Age |
48 years |
Gender |
female |
Ethnicity |
Caucasian |
Images |
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Derivation |
The line was established in December 1986 from lymph node metastasis of lung. |
Clinical Data |
48 years
Caucasian
female
The patient was a smoker.
60 pack years. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
Cryopreservation |
Culture medium, 95%; DMSO, 5% |
Culture Conditions |
Temperature: 37°C |
STR Profile |
Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 10,12 D5S818: 9 D7S820: 8,9 THO1: 9 TPOX: 8,11 vWA: 16,17 |
Name of Depositor |
AF Gazdar, JD Minna |
Deposited As |
Homo sapiens |
Year of Origin |
1986 |
References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
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